...
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
3 | 5X Kapa HiFi Buffer | 16001700 | 48005100 |
0.45 | 10M dNTPs | 16001700 | 720765 |
0.3 | Kapa HiFi HotStart DNA Pol | 16001700 | 480510 |
7.25 | HPLC H2O | 16001700 | 1160012325 |
11 | Total Volume | 16001700 | 1760018700 |
Add 11 ul to each well of a hard shell, full skirt plate. Seal with bubble strips and store in refrigerator until needed label “NovaSeq4 PCR”. (If doing manually, one needs 2 ul of template and 2 ul of the primers).
...
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
3 | 5X Kapa HiFi Buffer | 16001700 | 48005100 |
0.45 | 10M dNTPs | 16001700 | 720765 |
0.3 | Kapa HiFi HotStart DNA Pol | 16001700 | 480510 |
7.25 | HPLC H2O | 16001700 | 1160012325 |
11 | Total Volume | 16001700 | 1760018700 |
Add 11 ul to each well of a hard shell, full skirt plate. Seal with bubble strips and store in refrigerator until needed labeled “NovaSeq4 PCR”. (If doing manually, one needs 2 ul of template and 2 ul of the primers).
...