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1000/Results = ul of Pool to Add
1000/25 = 40uL of Pool to Add
1000 - ul of Pool to Add = ul of “10 mM Tris 8.5” to Add
1000- 40 = 960uL of 10mM Tris 8.5
Dilute 1 nM full pool to loading concentration of 50 pM:
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