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Average results for the following plates (column 3):
4AH1_16S: 11.86 nanomoles
4AH1_ITS: 15.74 nanomoles
4AH2_16S: 36.27 nanomoles
4AH2_ITS: 43.5 nanomoles
4PA1_16S: 4.72 nanomoles
4PA1_ITS: 22.76 nanomoles
4PA2_16S: 8.58 nanomoles
4PA2_ITS: 84.66 nanomoles
Results from the first qPCR plate look good. We will continue by checking one pooled plate on the iSeq for further validation. Full qPCR results are below:
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MasterMix (make for 16 plates in 50mL conical tube)
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