...
4SC1_ITS:
4SC2_16S:
4SC2_ITS:
MasterMix (make for
...
24 plates in 50mL conical tube)
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
3 | 5X Kapa HiFi Buffer | 2500 | 7500 |
0.45 | 10M dNTPs | 2500 | 1125 |
0.3 | Kapa HiFi HotStart DNA Pol | 2500 | 750 |
7.25 | HPLC H2O | 2500 | 18125 |
11 | Total Volume | 2500 | 27500 |
...