...
Reagent | ul/rxn | rxns | ul needed |
|---|---|---|---|
H2O | 9.52 | 2502202380 | 2094.4 |
5x iProof buffer | 4 | 250220 | 1000880 |
10 mM dNTPs | 0.4 | 250220 | 10088 |
50 mM MgCl2 | 0.4 | 250220 | 10088 |
5 uM Illumina Primers | 1.33 | 250220 | 332292.56 |
iProof TAQ | 0.2 | 250220 | 5044 |
DMSO | 0.15 | 250220 | 37.533 |
total | 16 | 250 | 4000 |
...
220 | 3520 |
Seal, Vortex, Spin down, and then run on Thermocycler program: GBS1:
...