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Add 4 ul of template, pool, or standards to each well:

	1	2	3	4	5	6	7	8	9	10	11	12
	1	2	3	4	5	6	7	8	9	10	11	12
A	GBS10-11 PCR_A Col_1	GBS10-11 PCR_A Col_6	GBS10-11 PCR_A Col_12	GBS10-11 PCR_B Col_1	GBS10-11 PCR_B Col_6	GBS10-11 PCR_B Col_12	GBS10-11 PCR_A Pool			NTC	NTC	NTC
B	GBS10-11 PCR_A Col_1	GBS10-11 PCR_A Col_6	GBS10-11 PCR_A Col_12	GBS10-11 PCR_B Col_1	GBS10-11 PCR_B Col_6	GBS10-11 PCR_B Col_12	GBS10-11 PCR_A Pool			0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	GBS10-11 PCR_A Col_1	GBS10-11 PCR_A Col_6	GBS10-11 PCR_A Col_12	GBS10-11 PCR_B Col_1	GBS10-11 PCR_B Col_6	GBS10-11 PCR_B Col_12	GBS10-11 PCR_B Pool			0.002 pM Std	0.002 pM Std	0.002 pM Std
D	GBS10-11 PCR_A Col_1	GBS10-11 PCR_A Col_6	GBS10-11 PCR_A Col_12	GBS10-11 PCR_B Col_1	GBS10-11 PCR_B Col_6	GBS10-11 PCR_B Col_12	GBS10-11 PCR_B Pool			0.02 pM Std	0.02 pM Std	0.02 pM Std
E	GBS10-11 PCR_A Col_1	GBS10-11 PCR_A Col_6	GBS10-11 PCR_A Col_12	GBS10-11 PCR_B Col_1	GBS10-11 PCR_B Col_6	GBS10-11 PCR_B Col_12	GBS10-11 Pool			0.2 pM Std	0.2 pM Std	0.2 pM Std
F	GBS10-11 PCR_A Col_1	GBS10-11 PCR_A Col_6	GBS10-11 PCR_A Col_12	GBS10-11 PCR_B Col_1	GBS10-11 PCR_B Col_6	GBS10-11 PCR_B Col_12	GBS10-11 Pool			2 pM Std	2 pM Std	2 pM Std
G	GBS10-11 PCR_A Col_1	GBS10-11 PCR_A Col_6	GBS10-11 PCR_A Col_12	GBS10-11 PCR_B Col_1	GBS10-11 PCR_B Col_6	GBS10-11 PCR_B Col_12				20 pM Std	20 pM Std	20 pM Std
H	GBS10-11 PCR_A Col_1	GBS10-11 PCR_A Col_6	GBS10-11 PCR_A Col_12	GBS10-11 PCR_B Col_1	GBS10-11 PCR_B Col_6	GBS10-11 PCR_B Col_12

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