We are expecting little to no difference. So, we will proceed with actual template, but the smaller PCR pool. We will compare via qPCR and bioanalyzer.
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Average quantities observed for all products:
GBS10-11 PCR_A Col_1: 78.13 nanomoles (thermocycler)
GBS10-11 PCR_A Col_6: 85.33 nanomoles (thermocycler)
GBS10-11 PCR_A Col_12: 75.57 nanomoles (thermocycler)
GBS10-11 PCR_B Col_1: 96.37 nanomoles (incubator)
GBS10-11 PCR_B Col_6: 78.72 nanomoles (incubator)
GBS10-11 PCR_B Col_12: 80.08 nanomoles (incubator)
GBS10-11 PCR_A Pool:Pool: 95.64 nanomoles (thermocycler)
GBS10-11 PCR_B Pool: 110.7 nanomoles (incubator)
GBS10-11 Pool: 95.91 nanomoles
GBS1-4 PCR_A Col_1: 87.22 nanomoles (repeated from previous qPCR)
GBS1-4 PCR_A Col_1:GBS1-4 PCR_B Col_1:B Col_1: 81.87 nanomoles (repeated from previous qPCR)
The full result report can be viewed below:
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Bioanalyzer
BioAnalyzer Chip Set up:
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Well 12: GBS10-11 PCR_B E11
Gel:
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Electropherogram:
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EPG Pools:
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EPG Wells 4-8:
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EPG Wells 9-12:
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