We are expecting little to no difference. So, we will proceed with actual template, but the smaller PCR pool. We will compare via qPCR and bioanalyzer.
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Cover, vortex, and spin plates. Incubate thermocycler plate at 16C for 6 hours. Incubate incubator plate at RT for 2 hours.
Add 189 120 ul of low EDTA TE store at 4C for a month or -20C for longer.
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GBS10-11 PCR_A Col_1: 78.13 nanomoles (thermocycler)
GBS10-11 PCR_A Col_6: 85.33 nanomoles (thermocycler)
GBS10-11 PCR_A Col_12: 75.57 nanomoles (thermocycler)
GBS10-11 PCR_B Col_1: 96.37 nanomoles (incubator)
GBS10-11 PCR_B Col_6: 78.72 nanomoles (incubator)
GBS10-11 PCR_B Col_12: 80.08 nanomoles (incubator)
GBS10-11 PCR_A Pool: 95.64 nanomoles (thermocycler)
GBS10-11 PCR_B Pool: 110.7 nanomoles (incubator)
GBS10-11 Pool: 95.91 nanomoles
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