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Status (9 10 March 2021)

  • Data arrived by mail on 24 February 2021. Alex Buerkle is processing the data to merged reads, and otu tablesThe otutables should be ready for users to work with.

Table of Contents

Demultiplexing and splitting

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In /project/microbiome/data/seq/cu_24feb21novaseq4/otu, I ran run_slurm_mkotu.pl, which I modified to also pick up the joined reads (in addition the merged reads).

This is where I am as of (the otu table generation has been launched).

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Make coligo table

In /project/microbiome/data/seq/psomagen_6mar20/coligoISD, /project/microbiome/data/seq/psomagen_26may20/coligoISD, and /project/microbiome/data/seq/psomagen_29jan21novaseq1c/coligoISD, there are 16S and ITS directories for all projects. These contain a file named coligoISDtable.txt with counts of the coligos and the ISD found in the trimmed forward reads, per sample. I did not do the fanciness I did for the otutable generation with project renaming (see above).