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Dilution and Prep for iSeq Sequencing

The aliquot of Pool2 size selected for the 300-450 bp range without dilution of initial pool qPCRs a concentration of 10.6 nM.

1. Dilute non-diluted pool to 1 nM: Add 9.5 ul Pool 2 to 90.5 ul TE.

2. Dilute to loading concentration: Add 10 ul from above to 90 ul 10u Tris

3. Equilibrate FlowCell to RT ~10 minutes

4. Load 20ul onto iSeq cartridge

5. Start Run