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Reagent | ul/rxn | rxns | ul needed |
|---|---|---|---|
5X KAPA HiFi HotStart PCR Buffer | 3 | 45 | 135 |
10M dNTPs | 0.45 | 45 | 20.3 |
Kapa HiFi HotStart DNA Pol | 0.3 | 45 | 13.5 |
HPLC H2O | 7.25 | 45 | 326.2 |
Total Volume | 11 | 45 | 495 |
Add 2 ul appropriate 2 1 uM paired primers Molecular IDentifier (MID) Plate:______________
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