...
Place sample plate back on magnet for 5 minutes or until all wells are cleared.
Transfer 40 ul to a clean PCR plate.
Check molar concentration via qPCR:
Dilute Samples 1:1000
Include NTC and Standards (20 pm, 2 pm, 0.2, 0.02pm, 0.002pm, 0.0002pm)
ul/rxn | Reagent |
|---|---|
10 ul | KAPA SYBR FAST qPCR MM (2X) |
2 ul | Primer Premix (10X) |
4 ul | Ultra Pure Water |
16 ul | Total Volume |
qPCR
Temp C | Cycles | Time |
|---|---|---|
94 | 1X | 0:01 |
95 | 1X | 5:00 |
95 | 32X | 0:30 |
60 | 1:00 |
If necessary, concentrate via Vacuum Concentrator (SpeedVac DNA130)