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	1	2	3	4	5	6	7	8	9	10	11	12
A	ALF_END_PLT1_16S_3B	ALF_END_PLT3_16S_3B	ALF_END_PLT5_16S_3B	ALF_END_PLT7_16S_3B								NTC
B	ALF_END_PLT1_16S_9E9H	ALF_END_PLT3_16S_9E9H	ALF_END_PLT5_16S_9E9H	ALF_END_PLT7_16S_9E9H							04.0002 pM Std	0.0002 pM Std
C	ALF_END_PLT1_ITS_3B	ALF_END_PLT3_ITS_3B	ALF_END_PLT5_ITS_3B	ALF_END_PLT7_ITS_3B							0.002 pM Std	0.002 pM Std
D	ALF_END_PLT1_ITS_9E9H	ALF_END_PLT3_ITS_9E9H	ALF_END_PLT5_ITS_9E9H	ALF_END_PLT7_ITS_9E9H							0.02 pM Std	0.02 pM Std
E	ALF_END_PLT2_16S_3B	ALF_END_PLT4_16S_3B	ALF_END_PLT6_16S_3B	ALF_END_PLT8_16S_3B							0.2 pM Std	0.2 pM Std
F	ALF_END_PLT2_16S_9E9H	ALF_END_PLT4_16S_9E9H	ALF_END_PLT6_16S_9E9H	ALF_END_PLT8_16S_9E9H							2 pM Std	2 pM Std
G	ALF_END_PLT2_ITS_3B	ALF_END_PLT4_ITS_3B	ALF_END_PLT6_ITS_3B	ALF_END_PLT8_ITS_3B							20 pM Std	20 pM Std
H	ALF_END_PLT2_ITS_9E9H	ALF_END_PLT4_ITS_9E9H	ALF_END_PLT6_ITS_9E9H	ALF_END_PLT8_ITS_9E9H

Add 16 ul of Illumina Library Quantification MasterMix to each well:

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	1	2	3	4	5	6	7	8	9	10	11	12
A	ALF_END_PLT1_16S_3B	ALF_END_PLT3_16S_3B	ALF_END_PLT5_16S_3B	ALF_END_PLT7_16S_3B						NTC	NTC	NTC
B	ALF_END_PLT1_16S_9E9H	ALF_END_PLT3_16S_9E9H	ALF_END_PLT5_16S_9E9H	ALF_END_PLT7_16S_9E9H						0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	ALF_END_PLT1_ITS_3B	ALF_END_PLT3_ITS_3B	ALF_END_PLT5_ITS_3B	ALF_END_PLT7_ITS_3B						0.002 pM Std	0.002 pM Std	0.002 pM Std
D	ALF_END_PLT1_ITS_9E9H	ALF_END_PLT3_ITS_9E9H	ALF_END_PLT5_ITS_9E9H	ALF_END_PLT7_ITS_9E9H						0.02 pM Std	0.02 pM Std	0.02 pM Std
E	ALF_END_PLT2_16S_3B	ALF_END_PLT4_16S_3B	ALF_END_PLT6_16S_3B	ALF_END_PLT8_16S_3B						0.2 pM Std	0.2 pM Std	0.2 pM Std
F	ALF_END_PLT2_16S_9E9H	ALF_END_PLT4_16S_9E9H	ALF_END_PLT6_16S_9E9H	ALF_END_PLT8_16S_9E9H						2 pM Std	2 pM Std	2 pM Std
G	ALF_END_PLT2_ITS_3B	ALF_END_PLT4_ITS_3B	ALF_END_PLT6_ITS_3B	ALF_END_PLT8_ITS_3B						20 pM Std	20 pM Std	20 pM Std
H	ALF_END_PLT2_ITS_9E9H	ALF_END_PLT4_ITS_9E9H	ALF_END_PLT6_ITS_9E9H	ALF_END_PLT8_ITS_9E9H

Results:

Average results for the following plates:

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	1	2	3	4	5	6	7	8	9	10	11	12
A					ALF_EPI_PLT1_16S_3B	ALF_EPI_PLT3_16S_3B	ALF_EPI_PLT5_16S_3B	ALF_EPI_PLT7_16S_3B		NTC	NTC	NTC
B					ALF_EPI_PLT1_16S_9E9H	ALF_EPI_PLT3_16S_9E9H	ALF_EPI_PLT5_16S_9E9H	ALF_EPI_PLT7_16S_9E9H		0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C					ALF_EPI_PLT1_ITS_3B	ALF_EPI_PLT3_ITS_3B	ALF_EPI_PLT5_ITS_3B	ALF_EPI_PLT7_ITS_3B		0.002 pM Std	0.002 pM Std	0.002 pM Std
D					ALF_EPI_PLT1_ITS_9E9H	ALF_EPI_PLT3_ITS_9E9H	ALF_EPI_PLT5_ITS_9E9H	ALF_EPI_PLT7_ITS_9E9H		0.02 pM Std	0.02 pM Std	0.02 pM Std
E					ALF_EPI_PLT2_16S_3B	ALF_EPI_PLT4_16S_3B	ALF_EPI_PLT6_16S_3B	ALF_EPI_PLT8_16S_3B		0.2 pM Std	0.2 pM Std	0.2 pM Std
F					ALF_EPI_PLT2_16S_9E9H	ALF_EPI_PLT4_16S_9E9H	ALF_EPI_PLT6_16S_9E9H	ALF_EPI_PLT8_16S_9E9H		2 pM Std	2 pM Std	2 pM Std
G					ALF_EPI_PLT2_ITS_3B	ALF_EPI_PLT4_ITS_3B	ALF_EPI_PLT6_ITS_3B	ALF_EPI_PLT8_ITS_3B		20 pM Std	20 pM Std	20 pM Std
H					ALF_EPI_PLT2_ITS_9E9H	ALF_EPI_PLT4_ITS_9E9H	ALF_EPI_PLT6_ITS_9E9H	ALF_EPI_PLT8_ITS_9E9H

Add 16 ul of Illumina Library Quantification MasterMix to each well:

...

	1	2	3	4	5	6	7	8	9	10	11	12
A	ALF_EPI_PLT1_16S_3B	ALF_EPI_PLT3_16S_3B	ALF_EPI_PLT5_16S_3B	ALF_EPI_PLT7_16S_3B						NTC	NTC	NTC
B	ALF_EPI_PLT1_16S_9E9H	ALF_EPI_PLT3_16S_9E9H	ALF_EPI_PLT5_16S_9E9H	ALF_EPI_PLT7_16S_9E9H						0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	ALF_EPI_PLT1_ITS_3B	ALF_EPI_PLT3_ITS_3B	ALF_EPI_PLT5_ITS_3B	ALF_EPI_PLT7_ITS_3B						0.002 pM Std	0.002 pM Std	0.002 pM Std
D	ALF_EPI_PLT1_ITS_9E9H	ALF_EPI_PLT3_ITS_9E9H	ALF_EPI_PLT5_ITS_9E9H	ALF_EPI_PLT7_ITS_9E9H						0.02 pM Std	0.02 pM Std	0.02 pM Std
E	ALF_EPI_PLT2_16S_3B	ALF_EPI_PLT4_16S_3B	ALF_EPI_PLT6_16S_3B	ALF_EPI_PLT8_16S_3B						0.2 pM Std	0.2 pM Std	0.2 pM Std
F	ALF_EPI_PLT2_16S_9E9H	ALF_EPI_PLT4_16S_9E9H	ALF_EPI_PLT6_16S_9E9H	ALF_EPI_PLT8_16S_9E9H						2 pM Std	2 pM Std	2 pM Std
G	ALF_EPI_PLT2_ITS_3B	ALF_EPI_PLT4_ITS_3B	ALF_EPI_PLT6_ITS_3B	ALF_EPI_PLT8_ITS_3B						20 pM Std	20 pM Std	20 pM Std
H	ALF_EPI_PLT2_ITS_9E9H	ALF_EPI_PLT4_ITS_9E9H	ALF_EPI_PLT6_ITS_9E9H	ALF_EPI_PLT8_ITS_9E9H

Results:

Average results for the following plates:

...

	1	2	3	4	5	6	7	8	9	10	11	12
A	ALF_GBS_PLT1_16S_3B	ALF_GBS_PLT3_16S_3B	ALF_GBS_PLT5_16S_3B	ALF_GBS_PLT7_16S_3B	ALF_GBS_PLT9_16S_3B	ALF_GBS_PLT11_16S_3B				NTC	NTC	NTC
B	ALF_GBS_PLT1_16S_9E9H	ALF_GBS_PLT3_16S_9E9H	ALF_GBS_PLT5_16S_9E9H	ALF_GBS_PLT7_16S_9E9H	ALF_GBS_PLT9_16S_9E9H	ALF_GBS_PLT11_16S_9A				0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	ALF_GBS_PLT1_ITS_3B	ALF_GBS_PLT3_ITS_3B	ALF_GBS_PLT5_ITS_3B	ALF_GBS_PLT7_ITS_3B	ALF_GBS_PLT9_ITS_3B	ALF_GBS_PLT11_ITS_3B				0.002 pM Std	0.002 pM Std	0.002 pM Std
D	ALF_GBS_PLT1_ITS_9E9H	ALF_GBS_PLT3_ITS_9E9H	ALF_GBS_PLT5_ITS_9E9H	ALF_GBS_PLT7_ITS_9E9H	ALF_GBS_PLT9_ITS_9E9H	ALF_GBS_PLT11_ITS_9A				0.02 pM Std	0.02 pM Std	0.02 pM Std
E	ALF_GBS_PLT2_16S_3B	ALF_GBS_PLT4_16S_3B	ALF_GBS_PLT6_16S_3B	ALF_GBS_PLT8_16S_3B	ALF_GBS_PLT10_16S_3B					0.2 pM Std	0.2 pM Std	0.2 pM Std
F	ALF_GBS_PLT2_16S_9E9H	ALF_GBS_PLT4_16S_9E9H	ALF_GBS_PLT6_16S_9E9H	ALF_GBS_PLT8_16S_9E9H	ALF_GBS_PLT10_16S_9E9H					2 pM Std	2 pM Std	2 pM Std
G	ALF_GBS_PLT2_ITS_3B	ALF_GBS_PLT4_ITS_3B	ALF_GBS_PLT6_ITS_3B	ALF_GBS_PLT8_ITS_3B	ALF_GBS_PLT10_ITS_3B					20 pM Std	20 pM Std	20 pM Std
H	ALF_GBS_PLT2_ITS_9E9H	ALF_GBS_PLT4_ITS_9E9H	ALF_GBS_PLT6_ITS_9E9H	ALF_GBS_PLT8_ITS_9E9H	ALF_GBS_PLT10_ITS_9E9H

Add 16 ul of Illumina Library Quantification MasterMix to each well:

...

	1	2	3	4	5	6	7	8	9	10	11	12
A	ALF_GBS_PLT1_16S_3B	ALF_GBS_PLT3_16S_3B	ALF_GBS_PLT5_16S_3B	ALF_GBS_PLT7_16S_3B	ALF_GBS_PLT9_16S_3B	ALF_GBS_PLT11_16S_3B				NTC	NTC	NTC
B	ALF_GBS_PLT1_16S_9E9H	ALF_GBS_PLT3_16S_9E9H	ALF_GBS_PLT5_16S_9E9H	ALF_GBS_PLT7_16S_9E9H	ALF_GBS_PLT9_16S_9E9H	ALF_GBS_PLT11_16S_9A				0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	ALF_GBS_PLT1_ITS_3B	ALF_GBS_PLT3_ITS_3B	ALF_GBS_PLT5_ITS_3B	ALF_GBS_PLT7_ITS_3B	ALF_GBS_PLT9_ITS_3B	ALF_GBS_PLT11_ITS_3B				0.002 pM Std	0.002 pM Std	0.002 pM Std
D	ALF_GBS_PLT1_ITS_9E9H	ALF_GBS_PLT3_ITS_9E9H	ALF_GBS_PLT5_ITS_9E9H	ALF_GBS_PLT7_ITS_9E9H	ALF_GBS_PLT9_ITS_9E9H	ALF_GBS_PLT11_ITS_9A				0.02 pM Std	0.02 pM Std	0.02 pM Std
E	ALF_GBS_PLT2_16S_3B	ALF_GBS_PLT4_16S_3B	ALF_GBS_PLT6_16S_3B	ALF_GBS_PLT8_16S_3B	ALF_GBS_PLT10_16S_3B					0.2 pM Std	0.2 pM Std	0.2 pM Std
F	ALF_GBS_PLT2_16S_9E9H	ALF_GBS_PLT4_16S_9E9H	ALF_GBS_PLT6_16S_9E9H	ALF_GBS_PLT8_16S_9E9H	ALF_GBS_PLT10_16S_9E9H					2 pM Std	2 pM Std	2 pM Std
G	ALF_GBS_PLT2_ITS_3B	ALF_GBS_PLT4_ITS_3B	ALF_GBS_PLT6_ITS_3B	ALF_GBS_PLT8_ITS_3B	ALF_GBS_PLT10_ITS_3B					20 pM Std	20 pM Std	20 pM Std
H	ALF_GBS_PLT2_ITS_9E9H	ALF_GBS_PLT4_ITS_9E9H	ALF_GBS_PLT6_ITS_9E9H	ALF_GBS_PLT8_ITS_9E9H	ALF_GBS_PLT10_ITS_9E9H

Results:

Average results for the following plates:

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