| Info |
|---|
The four pools were mailed to the University of Colorado’s Genomics Core on 03/23/2021. Pools were ran on 03/30/21. Data uploaded 4/02/2021. |
...
Pool | Lane | PF Clusters | % of the | % Perfect | % One mismatch | Yield (Mbases) | % PF | % >= Q30 | Mean Quality |
|---|---|---|---|---|---|---|---|---|---|
3 | 1 | 482,619,189 | 100.00 | 100.00 | NaN | 52,605 | 75.61 | 89.39 | 34.89 |
4 | 2 | 427,134,565 | 100.00 | 100.00 | NaN | 46,558 | 66.91 | 87.90 | 34.62 |
Reagents and Ordering
- EcoR1 (20,000 units/ml)
- MseI (10,000 units/ml)
- T4 DNA ligase buffer (400,000 units/ml)
- DNA polymerase (BioRad iProof or KAPA HiFi)
- BSA (1 mg/ml)-We have this in 20 mg/ul
- 1 M NaCl-We have this at 5M
- DMSO
- EcoR1 adaptors (We need 1 uM MIDed in plates)
- Mse1 Adaptors (10 uM stock probably need to dilute from 100 uM)
- IIllpcr2 (10 uM working)
- Illpcr1 (10 uM working)
- Pool of above 2 primers (5 uM working of each)
...
Sequencing Pool | PCR Pool | PCR Pool |
|---|---|---|
seqPool1 | Pool1 | Pool2 |
seqPool2 | Pool3 | Pool4 |
seqPool3 | Pool5 | Pool6 |
Size Selection
We will utilize utilized the Pippin Prep machine on loan from the Ernest Lab, selecting for the range 300-450bp.