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| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | 5RM1_16S_Col1 | 5RM1_16S_Col4 | 5RM1_16S_Col8 | 5RM1_16S_Pool |
| NTC | NTC | NTC | ||||
B | 5RM1_16S_Col1 | 5RM1_16S_Col4 | 5RM1_16S_Col8 | 5RM1_16S_Pool |
| 0.0002 pM Std | 0.0002 pM Std | 0.0002 pM Std | ||||
C | 5RM1_16S_Col1 | 5RM1_16S_Col4 | 5RM1_16S_Col8 |
| 0.002 pM Std | 0.002 pM Std | 0.002 pM Std | |||||
D | 5RM1_16S_Col1 | 5RM1_16S_Col4 | 5RM1_16S_Col8 |
| 0.02 pM Std | 0.02 pM Std | 0.02 pM Std | |||||
E | 5RM1_16S_Col1 | 5RM1_16S_Col4 | 5RM1_16S_Col8 |
| 0.2 pM Std | 0.2 pM Std | 0.2 pM Std | |||||
F | 5RM1_16S_Col1 | 5RM1_16S_Col4 | 5RM1_16S_Col8 |
| 2 pM Std | 2 pM Std | 2 pM Std | |||||
G | 5RM1_16S_Col1 | 5RM1_16S_Col4 | 5RM1_16S_Col8 |
| 20 pM Std | 20 pM Std | 20 pM Std | |||||
H | 5RM1_16S_Col1 | 5RM1_16S_Col4 | 5RM1_16S_Col8 |
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Average results for the following plates:
5RM1_16S_Col1: 0.489 nanomoles
5RM1_16S_Col4: 0.882 nanomoles
5RM1_16S_Col8: 1.29 nanomoles
5RM1_16S_Pool: 2.88 nanomoles
Full result report can be viewed below:
| View file | ||
|---|---|---|
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iSeq Run:
Dilute to 1 nM based off qPCR results. qPCR results are in pM, but 1:1000 dilution used. The results are effectively in nM for pool.
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