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Add 5 ul 1 nM Pool to 85 ul “10 mM Tris 8.5” and 10 ul 50 pM PhiX
Remove iSeq 100 i1 Flow Cell from refrigerator 5’s crisper drawer and open white foil pack and allow to equilibrate to RT for 10-15 minutes.
Open “iSeq 100 i1 Reagent Cartridge v2”. Turn on iSeq100
Click on “Sequence”. Watch Video. Do what video tells you to do. Follow on screen instructions until run starts.
The sequencing run showed poor global statistics:
Statistic | Percentage |
|---|---|
%Q30 Read 1 | 74.6 |
%Q30 Read 2 | 65.8 |
%Clusters PF | 32.3 |
%Occupancy | 94 |
A double check of the 1nM pool dilution created for sequencing revealed it was actually 4.98 nM. We will repeat sequencing run.