Samples:
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Paper detailing target and base primers
Fwd Primer Base (WANDA): CAGCCGCGGTAATTCCAGCT
Alternatives for WANDA:
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NS31: TTGGAGGGCAAGTCTGGTGCC No dimerization potential per OligoCalc. ∼530 bp amplicon.
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Fwd Primer Base (AMV4.5NF): AAGCTCGTAGTTGAATTTCG
Rev Primer Base (AML2AMDGR): GAACCCAAACACTTTGGTTTCCCCCAACTATCCCTATTAATCA
200 sample multiplex required. Sticking to full columns for ease of handling, we will need 16 24 forward and 16 24 reverse primers for 256 576 possible combinations.
Design and sequences of 2-step sequencing primers.
Test Samples: rhizosphere extractions
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