Test samples were previously extracted.
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Row | Annealing Temperature |
|---|---|
A | 60 |
B | 57 |
C | 55 |
D | 53 |
E | 50 |
F | 48 |
G | 46 |
H | 44 |
MagBead Cleanup:
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Make 1:1000 dilutions of column 1,2,3 and 6,7,8 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate: (Will fill in with proper sample names.)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | P1_A | P2_A | P3_A | P4_A | S1_A | S2_A | S3_A | S4_A |
| NTC | NTC | NTC |
B |
| 0.0002 pM Std | 0.0002 pM Std | 0.0002 pM Std | ||||||||
C |
| 0.002 pM Std | 0.002 pM Std | 0.002 pM Std | ||||||||
D |
| 0.02 pM Std | 0.02 pM Std | 0.02 pM Std | ||||||||
E |
| 0.2 pM Std | 0.2 pM Std | 0.2 pM Std | ||||||||
F |
| 2 pM Std | 2 pM Std | 2 pM Std | ||||||||
G |
| 20 pM Std | 20 pM Std | 20 pM Std | ||||||||
H |
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