Test samples were previously extracted.
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Make 1:1000 dilutions of column 1,2,3 and 6,7,-8 from the PCR plates plate by adding 1 ul to 999 ul TE in a deep well plate: (Will fill in with proper sample names.)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| NTC | NTC | NTC |
B | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 0.0002 pM Std | 0.0002 pM Std | 0.0002 pM Std |
C | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 0.002 pM Std | 0.002 pM Std | 0.002 pM Std |
D | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 0.02 pM Std | 0.02 pM Std | 0.02 pM Std |
E | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 0.2 pM Std | 0.2 pM Std | 0.2 pM Std |
F | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 2 pM Std | 2 pM Std | 2 pM Std |
G | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 20 pM Std | 20 pM Std | 20 pM Std |
H | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
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