Test samples were previously extracted.
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Template Format:
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | P1 | P2 | P3 | P4 | S1 | S2 | S3 | S4 | B | P1 | P2 | P3 | P4 | S1 | S2 | S3 | S4 | C | P1 | P2 | P3 | P4 | S1 | S2 | S3 | S4 | D | P1 | P2 | P3 | P4 | S1 | S2 | S3 | S4 | E | P1 | P2 | P3 | P4 | S1 | S2 | S3 | S4 | F | P1 | P2 | P3 | P4 | S1 | S2 | S3 | S4 | G | P1 | P2 | P3 | P4 | S1 | S2 | S3 | S4 | H | P1 | P2 | P3 | P4 | S1 | S2 | S3 | S4P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) | ||||
B | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
C | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
D | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
E | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
F | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
G | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
H | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
Run on thermocycler program TRNL_T*:
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Make 1:1000 dilutions of column 1,2,3 and 6,7,8 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate: (Will fill in with proper sample names.)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | P1_A | P2_A | P3_A | P4_A | S1_A | S2_A | S3_A | S4_AP2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| NTC | NTC | NTC |
B | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 0.0002 pM Std | 0.0002 pM Std | 0.0002 pM Std | |||||||
C | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 0.002 pM Std | 0.002 pM Std | 0.002 pM Std | |||||||
D | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 0.02 pM Std | 0.02 pM Std | 0.02 pM Std | |||||||
E | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 0.2 pM Std | 0.2 pM Std | 0.2 pM Std | |||||||
F | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 2 pM Std | 2 pM Std | 2 pM Std | |||||||
G | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
| 20 pM Std | 20 pM Std | 20 pM Std | |||||||
H | P2 (B1) | P7 (G1) | P9 (A2) | P14 (G2) | GM2 (C3) | SB4 (H3) | SB1 (D4) | AT16 (F4) |
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Add 16 ul of Illumina Library Quantification MasterMix to each well:
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Add 4 ul of template, pool, or standards to each well:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | P1P2_AP2 | P7_A | P3P9_A | P4P14_A | S1GM2_A | S2SB4_A | S3SB1_A | S4AT16_A |
| NTC | NTC | NTC |
B | P2_B | P7_B | P9_B | P14_B | GM2_B | SB4_B | SB1_B | AT16_B |
| 0.0002 pM Std | 0.0002 pM Std | 0.0002 pM Std |
C | P2_C | P7_C | P9_C | P14_C | GM2_C | SB4_C | SB1_C | AT16_C |
| 0.002 pM Std | 0.002 pM Std | 0.002 pM Std |
D | P2_D | P7_D | P9_D | P14_D | GM2_D | SB4_D | SB1_D | AT16_D |
| 0.02 pM Std | 0.02 pM Std | 0.02 pM Std |
E | P2_E | P7_E | P9_E | P14_E | GM2_E | SB4_E | SB1_E | AT16_E |
| 0.2 pM Std | 0.2 pM Std | 0.2 pM Std |
F | P2_F | P7_F | P9_F | P14_F | GM2_F | SB4_F | SB1_F | AT16_F |
| 2 pM Std | 2 pM Std | 2 pM Std |
G | P2_G | P7_G | P9_G | P14_G | GM2_G | SB4_G | SB1_G | AT16_G |
| 20 pM Std | 20 pM Std | 20 pM Std |
H | P2_H | P7_H | P9_H | P14_H | GM2_H | SB4_H | SB1_H | AT16_H |
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Results:
Average results
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