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	1	2	3	4	5	6	7	8	9	10	11	12
A	ALF_GBS_PLT1_16S_3B	ALF_GBS_PLT3_16S_3B	ALF_GBS_PLT5_16S_3B	ALF_GBS_PLT7_16S_3B	ALF_GBS_PLT9_16S_3B	ALF_GBS_PLT11_16S_3B	ALF_PLT1_2020_SOIL_16S_3B			NTC	NTC	NTC
B	ALF_GBS_PLT1_16S_9H	ALF_GBS_PLT3_16S_9H	ALF_GBS_PLT5_16S_9H	ALF_GBS_PLT7_16S_9H	ALF_GBS_PLT9_16S_9H	ALF_GBS_PLT11_16S_9A	ALF_PLT1_2020_SOIL_16S_9H			0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	ALF_GBS_PLT1_ITS_3B	ALF_GBS_PLT3_ITS_3B	ALF_GBS_PLT5_ITS_3B	ALF_GBS_PLT7_ITS_3B	ALF_GBS_PLT9_ITS_3B	ALF_GBS_PLT11_ITS_3B	ALF_PLT1_2020_SOIL_ITS_3B			0.002 pM Std	0.002 pM Std	0.002 pM Std
D	ALF_GBS_PLT1_ITS_9H	ALF_GBS_PLT3_ITS_9H	ALF_GBS_PLT5_ITS_9H	ALF_GBS_PLT7_ITS_9H	ALF_GBS_PLT9_ITS_9H	ALF_GBS_PLT11_ITS_9A	ALF_PLT1_2020_SOIL_ITS_9H			0.02 pM Std	0.02 pM Std	0.02 pM Std
E	ALF_GBS_PLT2_16S_3B	ALF_GBS_PLT4_16S_3B	ALF_GBS_PLT6_16S_3B	ALF_GBS_PLT8_16S_3B	ALF_GBS_PLT10_16S_3B	ALF_PLT9_2020_EPI_16S_1B				0.2 pM Std	0.2 pM Std	0.2 pM Std
F	ALF_GBS_PLT2_16S_9H	ALF_GBS_PLT4_16S_9H	ALF_GBS_PLT6_16S_9H	ALF_GBS_PLT8_16S_9H	ALF_GBS_PLT10_16S_9H	ALF_PLT9_2020_EPI_16S_3H				2 pM Std	2 pM Std	2 pM Std
G	ALF_GBS_PLT2_ITS_3B	ALF_GBS_PLT4_ITS_3B	ALF_GBS_PLT6_ITS_3B	ALF_GBS_PLT8_ITS_3B	ALF_GBS_PLT10_ITS_3B	ALF_PLT9_2020_EPI_ITS_1B				20 pM Std	20 pM Std	20 pM Std
H	ALF_GBS_PLT2_ITS_9H	ALF_GBS_PLT4_ITS_9H	ALF_GBS_PLT6_ITS_9H	ALF_GBS_PLT8_ITS_9H	ALF_GBS_PLT10_ITS_9H	ALF_PLT9_2020_EPI_ITS_3H

Add 16 ul of Illumina Library Quantification MasterMix to each well:

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	1	2	3	4	5	6	7	8	9	10	11	12
A	ALF_GBS_PLT1_16S_3B	ALF_GBS_PLT3_16S_3B	ALF_GBS_PLT5_16S_3B	ALF_GBS_PLT7_16S_3B	ALF_GBS_PLT9_16S_3B	ALF_GBS_PLT11_16S_3B	ALF_PLT1_2020_SOIL_16S_3B			NTC	NTC	NTC
B	ALF_GBS_PLT1_16S_9H	ALF_GBS_PLT3_16S_9H	ALF_GBS_PLT5_16S_9H	ALF_GBS_PLT7_16S_9H	ALF_GBS_PLT9_16S_9H	ALF_GBS_PLT11_16S_9A	ALF_PLT1_2020_SOIL_16S_9H			0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	ALF_GBS_PLT1_ITS_3B	ALF_GBS_PLT3_ITS_3B	ALF_GBS_PLT5_ITS_3B	ALF_GBS_PLT7_ITS_3B	ALF_GBS_PLT9_ITS_3B	ALF_GBS_PLT11_ITS_3B	ALF_PLT1_2020_SOIL_ITS_3B			0.002 pM Std	0.002 pM Std	0.002 pM Std
D	ALF_GBS_PLT1_ITS_9H	ALF_GBS_PLT3_ITS_9H	ALF_GBS_PLT5_ITS_9H	ALF_GBS_PLT7_ITS_9H	ALF_GBS_PLT9_ITS_9H	ALF_GBS_PLT11_ITS_9A	ALF_PLT1_2020_SOIL_ITS_9H			0.02 pM Std	0.02 pM Std	0.02 pM Std
E	ALF_GBS_PLT2_16S_3B	ALF_GBS_PLT4_16S_3B	ALF_GBS_PLT6_16S_3B	ALF_GBS_PLT8_16S_3B	ALF_GBS_PLT10_16S_3B	ALF_PLT9_2020_EPI_16S_1B				0.2 pM Std	0.2 pM Std	0.2 pM Std
F	ALF_GBS_PLT2_16S_9H	ALF_GBS_PLT4_16S_9H	ALF_GBS_PLT6_16S_9H	ALF_GBS_PLT8_16S_9H	ALF_GBS_PLT10_16S_9H	ALF_PLT9_2020_EPI_16S_3H				2 pM Std	2 pM Std	2 pM Std
G	ALF_GBS_PLT2_ITS_3B	ALF_GBS_PLT4_ITS_3B	ALF_GBS_PLT6_ITS_3B	ALF_GBS_PLT8_ITS_3B	ALF_GBS_PLT10_ITS_3B	ALF_PLT9_2020_EPI_ITS_1B				20 pM Std	20 pM Std	20 pM Std
H	ALF_GBS_PLT2_ITS_9H	ALF_GBS_PLT4_ITS_9H	ALF_GBS_PLT6_ITS_9H	ALF_GBS_PLT8_ITS_9H	ALF_GBS_PLT10_ITS_9H	ALF_PLT9_2020_EPI_ITS_3H

Results:

Average results for the following plates:

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ALF_GBS_PLT11_16S:

ALF_GBS_PLT11_ITS:

ALF_PLT9_2020_EPI_16S:

ALF_PLT9_2020_EPI_ITS:

ALF_PLT1_2020_SOIL_16S:

ALF_PLT1_2020_SOIL_ITS:

The full result report can be viewed below: