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  • Add 11 ul to each well of a hard shell, full skirt plate. Add 2 uL of primers and 2uL of template to each well.

  • Primers: _______________ (fill in when primers arrive).TRNL01

Template Format:

1

2

3

4

5

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7

8

9

10

11

12

A

P2 (B1)

P7 (G1)

P9 (A2)

P14 (G2)

GM2 (C3)

SB4 (H3)

SB1 (D4)

AT16 (F4)

B

P2 (B1)

P7 (G1)

P9 (A2)

P14 (G2)

GM2 (C3)

SB4 (H3)

SB1 (D4)

AT16 (F4)

C

P2 (B1)

P7 (G1)

P9 (A2)

P14 (G2)

GM2 (C3)

SB4 (H3)

SB1 (D4)

AT16 (F4)

D

P2 (B1)

P7 (G1)

P9 (A2)

P14 (G2)

GM2 (C3)

SB4 (H3)

SB1 (D4)

AT16 (F4)

E

P2 (B1)

P7 (G1)

P9 (A2)

P14 (G2)

GM2 (C3)

SB4 (H3)

SB1 (D4)

AT16 (F4)

F

P2 (B1)

P7 (G1)

P9 (A2)

P14 (G2)

GM2 (C3)

SB4 (H3)

SB1 (D4)

AT16 (F4)

G

P2 (B1)

P7 (G1)

P9 (A2)

P14 (G2)

GM2 (C3)

SB4 (H3)

SB1 (D4)

AT16 (F4)

H

P2 (B1)

P7 (G1)

P9 (A2)

P14 (G2)

GM2 (C3)

SB4 (H3)

SB1 (D4)

AT16 (F4)

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