Test Plates
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Add 11 ul to each well of a hard shell, full skirt plate. Add 2 uL of primers and 2uL of template to each well.
Primers: _______________ (fill in when primers arrive).AMF06
Template Format:
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
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A | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 |
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B | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 |
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C | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 |
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D | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 |
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E | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 |
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F | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 |
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G | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 |
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H | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 | BSP1 C1 |
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Make 1:1000 dilutions of column 1,2,3 and 6,7,8 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate: (Will fill in with proper sample names.)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | S1_A | S2_A | S3_A | S4_A | S5_A | S6_A | S7_A | S8_A |
| NTC | NTC | NTC |
B |
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| 0.0002 pM Std | 0.0002 pM Std | 0.0002 pM Std |
C |
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| 0.002 pM Std | 0.002 pM Std | 0.002 pM Std |
D |
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| 0.02 pM Std | 0.02 pM Std | 0.02 pM Std |
E |
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| 0.2 pM Std | 0.2 pM Std | 0.2 pM Std |
F |
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| 2 pM Std | 2 pM Std | 2 pM Std |
G |
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| 20 pM Std | 20 pM Std | 20 pM Std |
H |
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