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Test Plates

...

  • Make 1:1000 dilutions of column 1,2,3 and 6,7,8 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate: (Will fill in with proper sample names.)

 

1

2

3

4

5

6

7

8

9

10

11

12

A

S1_A

S2_A

S3_A

S4_A

S5_A

S6_A

S7_A

S8_A

 

NTC

NTC

NTC

B

 

 

 

 

 

 

 

 

 

0.0002 pM Std

0.0002 pM Std

0.0002 pM Std

C

 

 

 

 

 

 

 

 

 

0.002 pM Std

0.002 pM Std

0.002 pM Std

D

 

 

 

 

 

 

 

 

 

0.02 pM Std

0.02 pM Std

0.02 pM Std

E

 

 

 

 

 

 

 

 

 

0.2 pM Std

0.2 pM Std

0.2 pM Std

F

 

 

 

 

 

 

 

 

 

2 pM Std

2 pM Std

2 pM Std

G

 

 

 

 

 

 

 

 

 

20 pM Std

20 pM Std

20 pM Std

H

 

 

 

 

 

 

 

 

 

 

 

 

...

 

1

2

3

4

5

6

7

8

9

10

11

12

A

S1_A

S2_A

S3_A

S4_A

S5_A

S6_A

S7_A

S8_A

 

NTC

NTC

NTC

B

 

 

 

 

 

 

 

 

 

0.0002 pM Std

0.0002 pM Std

0.0002 pM Std

C

 

 

 

 

 

 

 

 

 

0.002 pM Std

0.002 pM Std

0.002 pM Std

D

 

 

 

 

 

 

 

 

 

0.02 pM Std

0.02 pM Std

0.02 pM Std

E

 

 

 

 

 

 

 

 

 

0.2 pM Std

0.2 pM Std

0.2 pM Std

F

 

 

 

 

 

 

 

 

 

2 pM Std

2 pM Std

2 pM Std

G

 

 

 

 

 

 

 

 

 

20 pM Std

20 pM Std

20 pM Std

H

 

 

 

 

 

 

 

 

 

 

 

 

Results:

qPCR results

Average results