...
Step | Temp C | Cycles | Time |
---|---|---|---|
Denature | 95 | 1X | 10:00 |
Annealing | 95 | 50X | 0:30 |
Annealing** (Row E) | 50 | 50X | 0:30 |
Extension/Elongation | 72 | 50X | 2:00 |
Hold | 4 | 1X | 0:00 |
...
...
Step | Temp C | Cycles | Time |
---|---|---|---|
Denature | 95 | 1X | 10:00 |
Annealing | 95 | 50X | 0:30 |
Annealing** (Row E) | 50 | 50X | 0:30 |
Extension/Elongation | 72 | 50X | 2:00 |
Hold | 4 | 1X | 0:00 |
...