...
compressed all
sample_fastq/
files with pigz: usingsbatch /project/microbiome/data/seq/HMAX1/demultiplex/run_pigz.sh
moved fastq for all four blank samples (data are all in one file because names are collapsed; noted above) to a subfolder (
/project/microbiome/data/seq/HMAX1/demultiplex/sample_fastq/blanks
), to get them out of the way.started denovo assembly in
/gscratch/buerkle/data/HMAX1/denovo
Completed first step for dDocent . and am running cd-hit -est nextfor 96% minimum match. Will also run 92/98.
To do:
...
Summarize the parse report files in /gscratch with some code to iterate over all the individual reports and get an overall count.
variant calling
...