qPCR
Make 1:1000 dilutions of all samples using 2 ml tubes
Add 16 ul of Illumina Library Quantification MasterMix to each well:
...
Sample Name | Detector | Task | Ct | StdDev Ct | Qty | Mean Qty | StdDev Qty |
|---|---|---|---|---|---|---|---|
LRII.5_Red_Pool | Unknown | Unknown | 9.31 | 0.605 | 16.65 | 12.97 | 5.204 |
LRII.5_Red_Pool | Unknown | Unknown | 10.17 | 0.605 | 9.29 | 12.97 | 5.204 |
LRII.5_Red_Pool | Unknown | Unknown | 5.7 | 2.734 | 196.45 | 106 | 74.13 |
RM_JAN22_II_Pool | Unknown | Unknown | 8.14 | 0.220 | 36.98 | 32.25 | 4.786 |
RM_JAN22_II_Pool | Unknown | Unknown | 8.34 | 0.220 | 32.36 | 32.25 | 4.786 |
RM_JAN22_II_Pool | Unknown | Unknown | 8.58 | 0.220 | 27.41 | 32.25 | 4.786 |
...
reads PhiX/Samples: 709274/1921404 = 0.37
expected Calculated PhiX/Samples: 0.16728Calculated
expected PhiX/Samples: 0.167