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New Control Samples:

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1

2

3

4

5

6

7

8

9

10

11

12

A

RA6

RA14

RA22

LL1A_J

WG3A_S

LY2A_S

MCA_2

MCA_4

MCA_8

NTC

NTC

NTC

B

RA7

RA15

RA23

LL1B_J

WG3B_S

LY2B_S

MCB_2

MCB_4

MCB_8

0.0002 pM Std

0.0002 pM Std

0.0002 pM Std

C

RA8

RA16

RA24

LL2A_J

WG4A_S

LY3B_S

MCC_2

MCC_4

MCC_8

0.002 pM Std

0.002 pM Std

0.002 pM Std

D

RA9

RA17

RA25

LL2B_J

WG4B_S

LY1A_S

MCD_2

MCD_4

MCD_8

0.02 pM Std

0.02 pM Std

0.02 pM Std

E

RA10

RA18

RA26

LL4A_J

WG2A_S

LY1B_S

MCA_2_clean

MCA_4_clean

MCA_8_clean

0.2 pM Std

0.2 pM Std

0.2 pM Std

F

RA11

RA19

RA27

LL4B_J

WG2B_S

LY4A_S

MCB_2_clean

MCB_4_clean

MCB_8_clean

2 pM Std

2 pM Std

2 pM Std

G

RA12

RA20

RA28

LL3A_J

WG1A_S

LY4B_S

MCC_2_clean

MCC_4_clean

MCC_8_clean

H

RA13

RA21

RA29

LL3B_J

WG1B_S

BLANK

MCD_2_clean

MCD_4_clean

MCD_8_clean

 

 

 

Plate

5LVD5

5LVD5

5LVD5

5DG5

5DG5

5DG5

Controls

Controls

Controls

Column

1

2

3

4

5

6

...

 

1

2

3

4

5

6

7

8

9

10

11

12

A

RA6

RA14

RA22

RA6

RA14

RA22

RA6

RA14

RA22

NTC

NTC

NTC

B

RA7

RA15

RA23

RA7

RA15

RA23

RA7

RA15

RA23

0.0002 pM Std

0.0002 pM Std

0.0002 pM Std

C

RA8

RA16

RA24

RA8

RA16

RA24

RA8

RA16

RA24

0.002 pM Std

0.002 pM Std

0.002 pM Std

D

RA9

RA17

RA25

RA9

RA17

RA25

RA9

RA17

RA25

0.02 pM Std

0.02 pM Std

0.02 pM Std

E

RA10

RA18

RA26

RA10

RA18

RA26

RA10

RA18

RA26

0.2 pM Std

0.2 pM Std

0.2 pM Std

F

RA11

RA19

RA27

RA11

RA19

RA27

RA11

RA19

RA27

2 pM Std

2 pM Std

2 pM Std

G

RA12

RA20

RA28

RA12

RA20

RA28

RA12

RA20

RA28

20 pM Std

20 pM Std

20 pM Std

H

RA13

RA21

RA29

RA13

RA21

RA29

RA13

RA21

RA29

 

 

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

LL1A_J

WG3A_S

LY2A_S

LL1A_J

WG3A_S

LY2A_S

LL1A_J

WG3A_S

LY2A_S

NTC

NTC

NTC

B

LL1B_J

WG3B_S

LY2B_S

LL1B_J

WG3B_S

LY2B_S

LL1B_J

WG3B_S

LY2B_S

0.0002 pM Std

0.0002 pM Std

0.0002 pM Std

C

LL2A_J

WG4A_S

LY3B_S

LL2A_J

WG4A_S

LY3B_S

LL2A_J

WG4A_S

LY3B_S

0.002 pM Std

0.002 pM Std

0.002 pM Std

D

LL2B_J

WG4B_S

LY1A_S

LL2B_J

WG4B_S

LY1A_S

LL2B_J

WG4B_S

LY1A_S

0.02 pM Std

0.02 pM Std

0.02 pM Std

E

LL4A_J

WG2A_S

LY1B_S

LL4A_J

WG2A_S

LY1B_S

LL4A_J

WG2A_S

LY1B_S

0.2 pM Std

0.2 pM Std

0.2 pM Std

F

LL4B_J

WG2B_S

LY4A_S

LL4B_J

WG2B_S

LY4A_S

LL4B_J

WG2B_S

LY4A_S

2 pM Std

2 pM Std

2 pM Std

G

LL3A_J

WG1A_S

LY4B_S

LL3A_J

WG1A_S

LY4B_S

LL3A_J

WG1A_S

LY4B_S

20 pM Std

20 pM Std

20 pM Std

H

LL3B_J

WG1B_S

BLANK

LL3B_J

WG1B_S

BLANK

LL3B_J

WG1B_S

BLANK

 

 

 

 

1

2

3

4

5

6

7

8

9

10

11

12

A

MCA_2

MCA_4

MCA_8

MCA_2

MCA_4

MCA_8

MCA_2

MCA_4

MCA_8

NTC

NTC

NTC

B

MCB_2

MCB_4

MCB_8

MCB_2

MCB_4

MCB_8

MCB_2

MCB_4

MCB_8

0.0002 pM Std

0.0002 pM Std

0.0002 pM Std

C

MCC_2

MCC_4

MCC_8

MCC_2

MCC_4

MCC_8

MCC_2

MCC_4

MCC_8

0.002 pM Std

0.002 pM Std

0.002 pM Std

D

MCD_2

MCD_4

MCD_8

MCD_2

MCD_4

MCD_8

MCD_2

MCD_4

MCD_8

0.02 pM Std

0.02 pM Std

0.02 pM Std

E

MCA_2_clean

MCA_4_clean

MCA_8_clean

MCA_2_clean

MCA_4_clean

MCA_8_clean

MCA_2_clean

MCA_4_clean

MCA_8_clean

0.2 pM Std

0.2 pM Std

0.2 pM Std

F

MCB_2_clean

MCB_4_clean

MCB_8_clean

MCB_2_clean

MCB_4_clean

MCB_8_clean

MCB_2_clean

MCB_4_clean

MCB_8_clean

2 pM Std

2 pM Std

2 pM Std

G

MCC_2_clean

MCC_4_clean

MCC_8_clean

MCC_2_clean

MCC_4_clean

MCC_8_clean

MCC_2_clean

MCC_4_clean

MCC_8_clean

20 pM Std

20 pM Std

20 pM Std

H

MCD_2_clean

MCD_4_clean

MCD_8_clean

MCD_2_clean

MCD_4_clean

MCD_8_clean

MCD_2_clean

MCD_4_clean

MCD_8_clean

 

 

 

Controls

Controls

Controls

Controls

Controls

Controls

Controls

Controls

Controls

...

Dilute to loading concentration with 20% PhiX.

Loading pool is:

74ul 78ul 10mM Tris HCL pH 8, 20 16 ul 50 pM PhiX, 6 ul from above pool.

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