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Version	Old Version 5	New Version 6
Changes made by	Shannon Harris	Gregg Randolph
Saved on	Mar 17, 2022	Mar 18, 2022

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This line was removed.
Formatting was changed.

New Control Samples:

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	1	2	3	4	5	6	7	8	9	10	11	12
A	RA6	RA14	RA22	LL1A_J	WG3A_S	LY2A_S	MCA_2	MCA_4	MCA_8	NTC	NTC	NTC
B	RA7	RA15	RA23	LL1B_J	WG3B_S	LY2B_S	MCB_2	MCB_4	MCB_8	0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	RA8	RA16	RA24	LL2A_J	WG4A_S	LY3B_S	MCC_2	MCC_4	MCC_8	0.002 pM Std	0.002 pM Std	0.002 pM Std
D	RA9	RA17	RA25	LL2B_J	WG4B_S	LY1A_S	MCD_2	MCD_4	MCD_8	0.02 pM Std	0.02 pM Std	0.02 pM Std
E	RA10	RA18	RA26	LL4A_J	WG2A_S	LY1B_S	MCA_2_clean	MCA_4_clean	MCA_8_clean	0.2 pM Std	0.2 pM Std	0.2 pM Std
F	RA11	RA19	RA27	LL4B_J	WG2B_S	LY4A_S	MCB_2_clean	MCB_4_clean	MCB_8_clean	2 pM Std	2 pM Std	2 pM Std
G	RA12	RA20	RA28	LL3A_J	WG1A_S	LY4B_S	MCC_2_clean	MCC_4_clean	MCC_8_clean
H	RA13	RA21	RA29	LL3B_J	WG1B_S	BLANK	MCD_2_clean	MCD_4_clean	MCD_8_clean
Plate	5LVD5	5LVD5	5LVD5	5DG5	5DG5	5DG5	Controls	Controls	Controls
Column	1	2	3	4	5	6

...

	1	2	3	4	5	6	7	8	9	10	11	12
A	RA6	RA14	RA22	RA6	RA14	RA22	RA6	RA14	RA22	NTC	NTC	NTC
B	RA7	RA15	RA23	RA7	RA15	RA23	RA7	RA15	RA23	0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	RA8	RA16	RA24	RA8	RA16	RA24	RA8	RA16	RA24	0.002 pM Std	0.002 pM Std	0.002 pM Std
D	RA9	RA17	RA25	RA9	RA17	RA25	RA9	RA17	RA25	0.02 pM Std	0.02 pM Std	0.02 pM Std
E	RA10	RA18	RA26	RA10	RA18	RA26	RA10	RA18	RA26	0.2 pM Std	0.2 pM Std	0.2 pM Std
F	RA11	RA19	RA27	RA11	RA19	RA27	RA11	RA19	RA27	2 pM Std	2 pM Std	2 pM Std
G	RA12	RA20	RA28	RA12	RA20	RA28	RA12	RA20	RA28	20 pM Std	20 pM Std	20 pM Std
H	RA13	RA21	RA29	RA13	RA21	RA29	RA13	RA21	RA29

	1	2	3	4	5	6	7	8	9	10	11	12
A	LL1A_J	WG3A_S	LY2A_S	LL1A_J	WG3A_S	LY2A_S	LL1A_J	WG3A_S	LY2A_S	NTC	NTC	NTC
B	LL1B_J	WG3B_S	LY2B_S	LL1B_J	WG3B_S	LY2B_S	LL1B_J	WG3B_S	LY2B_S	0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	LL2A_J	WG4A_S	LY3B_S	LL2A_J	WG4A_S	LY3B_S	LL2A_J	WG4A_S	LY3B_S	0.002 pM Std	0.002 pM Std	0.002 pM Std
D	LL2B_J	WG4B_S	LY1A_S	LL2B_J	WG4B_S	LY1A_S	LL2B_J	WG4B_S	LY1A_S	0.02 pM Std	0.02 pM Std	0.02 pM Std
E	LL4A_J	WG2A_S	LY1B_S	LL4A_J	WG2A_S	LY1B_S	LL4A_J	WG2A_S	LY1B_S	0.2 pM Std	0.2 pM Std	0.2 pM Std
F	LL4B_J	WG2B_S	LY4A_S	LL4B_J	WG2B_S	LY4A_S	LL4B_J	WG2B_S	LY4A_S	2 pM Std	2 pM Std	2 pM Std
G	LL3A_J	WG1A_S	LY4B_S	LL3A_J	WG1A_S	LY4B_S	LL3A_J	WG1A_S	LY4B_S	20 pM Std	20 pM Std	20 pM Std
H	LL3B_J	WG1B_S	BLANK	LL3B_J	WG1B_S	BLANK	LL3B_J	WG1B_S	BLANK

	1	2	3	4	5	6	7	8	9	10	11	12
A	MCA_2	MCA_4	MCA_8	MCA_2	MCA_4	MCA_8	MCA_2	MCA_4	MCA_8	NTC	NTC	NTC
B	MCB_2	MCB_4	MCB_8	MCB_2	MCB_4	MCB_8	MCB_2	MCB_4	MCB_8	0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	MCC_2	MCC_4	MCC_8	MCC_2	MCC_4	MCC_8	MCC_2	MCC_4	MCC_8	0.002 pM Std	0.002 pM Std	0.002 pM Std
D	MCD_2	MCD_4	MCD_8	MCD_2	MCD_4	MCD_8	MCD_2	MCD_4	MCD_8	0.02 pM Std	0.02 pM Std	0.02 pM Std
E	MCA_2_clean	MCA_4_clean	MCA_8_clean	MCA_2_clean	MCA_4_clean	MCA_8_clean	MCA_2_clean	MCA_4_clean	MCA_8_clean	0.2 pM Std	0.2 pM Std	0.2 pM Std
F	MCB_2_clean	MCB_4_clean	MCB_8_clean	MCB_2_clean	MCB_4_clean	MCB_8_clean	MCB_2_clean	MCB_4_clean	MCB_8_clean	2 pM Std	2 pM Std	2 pM Std
G	MCC_2_clean	MCC_4_clean	MCC_8_clean	MCC_2_clean	MCC_4_clean	MCC_8_clean	MCC_2_clean	MCC_4_clean	MCC_8_clean	20 pM Std	20 pM Std	20 pM Std
H	MCD_2_clean	MCD_4_clean	MCD_8_clean	MCD_2_clean	MCD_4_clean	MCD_8_clean	MCD_2_clean	MCD_4_clean	MCD_8_clean
	Controls	Controls	Controls	Controls	Controls	Controls	Controls	Controls	Controls

...

Dilute to loading concentration with 20% PhiX.

Loading pool is:

75ul 74ul 10mM Tris HCL pH 8, 20 ul 50 pM PhiX, 5 6 ul from above pool.