Page Comparison

Raw reads

We retrieved four files from the iSeq. One of these contains the 1x150bp 1x100bp reads. The file with the raw reads of interest are ( in /project/microbiomegtl/data_queueseq/seqdistribution/trout1/Trout1_*/rawreads).

1. Trout-Pool3_S1_L001Trout1_P1_S1_L001_R1_001.fastq.gz (24G) – 457,726,974 reads (1.5 GBytes uncompressed)

2. Trout1_P2_S2_L002_R1_001.fastq.gz (25G) – 457,726,974 reads (1.5 GBytes uncompressed)

3. Trout1_P3_S1_L001_R1_001.fastq.gz (39G) – 457,726,974 reads (1.5 GBytes uncompressed)

4. Trout1_P4_S2_L002_R1_001.fastq.gz (273M40G) – 457,726,974 reads (1.5 GBytes uncompressed)

gunzip Trout-Pool3_S1_L001/project/gtl/seq/raw/trout1/Trout1_1/rawreads/Trout1_P1_S1_L001_R1_001.fastq.gz

gunzip /project/gtl/seq/raw/trout1/Trout1_2/rawreads/Trout1_P2_S2_L002_R1_001.fastq.gz

gunzip /project/gtl/seq/raw/trout1/Trout1_3/rawreads/Trout1_P3_S1_L001_R1_001.fastq.gz

gunzip /project/gtl/seq/raw/trout1/Trout1_4/rawreads/Trout1_P4_S2_L002_R1_001.fastq.gz

Needs updating below 4-26-22

Demultiplexing

Split into 100000 line files

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Parse split files

/project/microbiomegtl/analyses/gtl/HMAX1/demultiplex/run_parsebarcodes_onSplitInput.pl

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