...
run_splitFastq_fwd.sh
and run_splitFastq_rev.sh
run splitFastq_manyInputfiles.pl
, which steps through the many pairs of files to split reads by sample and project, and place them in /project/gtl/data/raw/ALF1/16S/rawdata/sample_fastq/
Stopped here on 8-31-22
Calculate summary statistics on reads
In a sequence library’s rawdata/
directory (e.g., /project/gtl/data/raw/ALF1/ITS/rawdata/
) I made run_aggregate.sh
, to run aggregate_usearch_fastx_info.pl
with a slurm job. Summaries are written to summary_sample_fastq.csv
.
Stopped here on 8-31-22
Trim, merge and filter reads
...