Plate | 16S Primer | ITS Primer | AMF |
|---|---|---|---|
6LVD5 | 16S0A4 | ITS0A4 | AMF01 |
16S0B4 | ITS0B4 | AMF02 | |
6LVD6 | 16S0C4 | ITS0C4 | AMF03 |
16S0D4 | ITS0D4 | AMF04 | |
6LVD7 | 16S0E4 | ITS0E4 | AMF05 |
16S0F4 | ITS0F4 | AMF06 |
MasterMix (make for 16 plates in 50mL conical tube)
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
3 | 5X Kapa HiFi Buffer | 1400 | 4200 |
0.45 | 10M dNTPs | 1400 | 630 |
0.3 | Kapa HiFi HotStart DNA Pol | 1400 | 420 |
7.25 | HPLC H2O | 1400 | 10,150 |
11 | Total Volume | 1400 | 15400 |
Add 11 ul to each well of a hard shell, full skirt plate. Seal with bubble strips and store in refrigerator until needed label “NS6 PCR”. (If doing manually, one needs 2 ul of template and 2 ul of the primers).