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Plates in red have been extracted, PCRed, cleaned, quantified, and normalized by 7-15-22. Bolded red have been qPCRed.

NS6 Plates

6AC1	6AC2	6AC3
6BM1	6BM2
6LVD1	6LVD2	6LVD3	6LVD4
6RD1	6RD2	6RD3
6GTL1	6GTL2
6RD4	6RD5	6RD6	6RD7
6LVD5	~~6LVD6~~	~~6LVD7~~
MP002	MP003	MP006

MasterMix (make for 16 plates in 50mL conical tube)

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Add 4 ul of template, pool, or standards to each well:

	1	2	3	4	5	6	7	8	9	10	11	12
A	NTC	NTC	NTC	B	0.0002 pM Std	0.0002 pM Std	0.0002 pM Std	C6RD4_16S	6RD4_ITS	6RD5_16S	6RD5_ITS	6RD6_16S	6RD6_ITS	6RD7_16S	6RD7_ITS	6LVD5_16S	NTC	NTC	NTC
B	6RD4_16S	6RD4_ITS	6RD5_16S	6RD5_ITS	6RD6_16S	6RD6_ITS	6RD7_16S	6RD7_ITS	6LVD5_16S	0.0002 pM Std	0.0002 pM Std	0.0002 pM Std
C	6RD4_16S	6RD4_ITS	6RD5_16S	6RD5_ITS	6RD6_16S	6RD6_ITS	6RD7_16S	6RD7_ITS	6LVD5_16S	0.002 pM Std	0.002 pM Std	0.002 pM Std
D	6RD4_16S	6RD4_ITS	6RD5_16S	6RD5_ITS	6RD6_16S	6RD6_ITS	6RD7_16S	6RD7_ITS	6LVD5_16S	0.02 pM Std	0.02 pM Std	0.02 pM Std	E	0.02 pM Std
E	6RD4_16S	6RD4_ITS	6RD5_16S	6RD5_ITS	6RD6_16S	6RD6_ITS	6RD7_16S	6RD7_ITS	6LVD5_ITS	0.2 pM Std	0.2 pM Std	0.2 pM Std
F	6RD4_16S	6RD4_ITS	6RD5_16S	6RD5_ITS	6RD6_16S	6RD6_ITS	6RD7_16S	6RD7_ITS	6LVD5_ITS	2 pM Std	2 pM Std	2 pM Std	G2 pM Std
G	6RD4_16S	6RD4_ITS	6RD5_16S	6RD5_ITS	6RD6_16S	6RD6_ITS	6RD7_16S	6RD7_ITS	6LVD5_ITS	20 pM Std	20 pM Std	20 pM Std
H	6RD4_16S	6RD4_ITS	6RD5_16S	6RD5_ITS	6RD6_16S	6RD6_ITS	6RD7_16S	6RD7_ITS	6LVD5_ITS

Results:

Plate Name	Average Result (nanomoles)
6RD4_16S
6RD4_ITS
6RD5_16S
6RD5_ITS
6RD6_16S
6RD6_ITS
6RD7_16S
6RD7_ITS
6LVD5_16S_ITS

Full qPCR results below:

qPCR NovaSeq6_Set5

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