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Status (02 May 2022)

Table of Contents

Demultiplexing and splitting

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Code Block
mkdir -p /gscratch/grandol1/NS6/rawdata
cd /gscratch/grandol1/NS6/rawdata
unpigz --to-stdout /project/microbiome/data_queue/seq/NS6/rawdata/NovaSeq6_pool_1.fq.gz | split -l 16000000 -d --suffix-length=3 --additional-suffix=.fastq - NS6_R1_ ;
unpigz --to-stdout /project/microbiome/data_queue/seq/NS6/rawdata/NovaSeq6_pool_2.fq.gz | split -l 16000000 -d --suffix-length=3 --additional-suffix=.fastq - NS6_R2_

making 94 256 R1 files and 94 256 R2 files, with structured names (e.g., for the R1 set):

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