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unpigz --to-stdout /project/gtl/data/raw/2AMF3AMF/rawdata/2AMF3AMF_S1_L001_R1_001.fqfastq.gz | split -l 1000000 10000000 -d --suffix-length=3 --additional-suffix=.fastq - 2AMF3AMF_R1_ ;
unpigz --to-stdout /project/gtl/data/raw/2AMF3AMF/rawdata/2AMF3AMF_S1_L001_R2_001.fqfastq.gz | split -l 1000000 10000000 -d --suffix-length=3 --additional-suffix=.fastq - 2AMF3AMF_R2_

//project/gtl/data/raw/2AMF3AMF/rawdata/run_parse_count_onSplitInput.plabove step running 12-30-22

cd /project/gtl/data/raw/2AMF3AMF/rawdata

./run_splitFastq_fwd.sh

./run_splitFastq_rev.sh./run_aggregate.sh

cd /project/gtl/data/raw/2AMF3AMF/tfmergedreads

./run_slurm_mergereads.pl

cd /project/gtl/data/raw/2AMF3AMF/otu

./run_slurm_mkotu.pl