Setup Notes
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Normalize plate reader with Buffer AE for all plates
Quantify all plates
Check for samples above 150 ng/ul
Transfer 20 ul from all wells of a plate needing normalization to new VWR plate
Add 20 ul TE to all wells above 150 ng/ul
Restriction Digestion
(Keep MM and reaction plates on ice)
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Reagent | ul/rxn | rxns | ul needed (x 1.6) | |
5x Iproof buffer | 0.425 | 1152 | 784 | 163.2278 |
10 mM dNTPs | 0.4 | 1152 | 738 | 153.6262 |
Primers | 1.33 | 1152 | 2452 | 511869 |
Total | 2.155 | 1152 | 3973.82828 |
Then continue with the last four unlisted steps for GBS1 from above:
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