Setup Notes
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Normalize plate reader with Buffer AE for all plates
Quantify all plates
Check for samples above 150 ng/ul
Transfer 20 ul from all wells of a plate needing normalization to new VWR plate
Add 20 ul TE to all wells above 150 ng/ul
Restriction Digestion
(Keep MM and reaction plates on ice)
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Add 1 ul of EcoR1 Adaptors with 96-channel and Track which template plate gets which MID plate.
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Template
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EcoR1 MID plate
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Pool Plate
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Template | EcoR1 MID plate | Pool Plate | Library |
|---|---|---|---|
WRosenthal_Trout_plate12 | 1 | 2TroutRedoPool1 | 1and2 |
WRosenthal_Trout_plate16 | 2 | 2TroutRedoPool1 | 1and2 |
WRosenthal_Trout_plate18 | 3 | 2TroutRedoPool2 | 1and2 |
Wrosenthal_PM_YCT_plate2 | 4 | 2TroutRedoPool2 | 1and2 |
MT_YCT_RangewideRADs_plate01 | 5 | 2TroutRedoPool3 | 3and4 |
Wrosenthal_GY21_YCT_tray6 | 6 | 2TroutRedoPool3 | 3and4 |
WY_YCT_2021_plate14 | 7 | 2TroutRedoPool4 | 3and4 |
Wrosenthal_PM_YCT_plate1 | 8 | 2TroutRedoPool4 | 3and4 |
WRosenthal_Trout_plate9 | 1 | 2TroutRedoPool5 | 5and6 |
WRosenthal_Trout_plate14 | 2 | 2TroutRedoPool5 | 5and6 |
WRosenthal_Trout_plate20 | 3 | 2TroutRedoPool6 | 5and6 |
WRosenthal_Trout_plate21 | 4 | 2TroutRedoPool6 | 5and6 |
WY_YCT_2021_plate10 | 5 | 2TroutRedoPool7 | 7and8 |
WY_YCT_2021_plate13 | 6 | 2TroutRedoPool7 | 7and8 |
WRosenthal_Trout_plate10 | 1 | 2TroutRedoPool8 | 7and8 |
WRosenthal_Trout_plate13 | 2 | 2TroutRedoPool8 | 7and8 |
WRosenthal_Trout_plate11 | 1 | 2TroutRedoPool9 | 9and12 |
MT_YCT_RangewideRADs_plate03 | 5 | 2TroutRedoPool9 | 9and12 |
WY_YCT_2021_plate11 | 6 | 2TroutRedoPool9 | 9and12 |
MT_WCT_plate15 | |||
WRosenthal_Trout_plate19 | 3 | 2TroutRedoPool10 | 10and14 |
WY_YCT_2021_plate04 | 7 | 2TroutRedoPool11 | 11and13 |
WY_YCT_2021_plate09 | 5 | 2TroutRedoPool11 | 11and13 |
Wrosenthal_GY21_YCT_tray3 | 7 | 2TroutRedoPool12 | 9and12 |
Wrosenthal_GY21_YCT_tray7 | 8 | 2TroutRedoPool12 | 9and12 |
WRosenthal_Trout_plate17 | 3 | 2TroutRedoPool13 | 11and13 |
WRosenthal_Trout_plate24 | 4 | 2TroutRedoPool13 | 11and13 |
WRosenthal_Trout_plate15 | 2 | 2TroutRedoPool14 | 10and14 |
WRosenthal_Trout_plate22 | 4 | 2TroutRedoPool14 | 10and14 |
WRosenthal_Trout_plate25 | 8 | 2TroutRedoPool15 | 15and16 |
Wrosenthal_GY21_YCT_tray4 | 6 | 2TroutRedoPool15 | 15and16 |
Wrosenthal_GY21_YCT_tray1 | 7 | 2TroutRedoPool16 | 15and16 |
Label reaction plates with MID plate used.
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