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Setup Notes

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  • Normalize plate reader with Buffer AE for all plates

  • Quantify all plates

  • Check for samples above 150 ng/ul

  • Transfer 20 ul from all wells of a plate needing normalization to new VWR plate

  • Add 20 ul TE to all wells above 150 ng/ul

Restriction Digestion

(Keep MM and reaction plates on ice)

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