...
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
3 | 5X Kapa HiFi Buffer | 384 | 1320 |
0.45 | 10M dNTPs | 384 | 198 |
0.37.5 | Kapa HiFi HotStart DNA Pol2X | 384120 | 132900 |
74.255 | HPLC H2O | 384120 | 3190540 |
1112 | Total Volume | 384 | 48401440 |
Add 11 ul to each well of a hard shell, full skirt plate. Add 2 1 uL of 0.5 uM primers and 2uL of template to each well.
Primers:
...