...
Reagent | ul/rxn | rxns | ul needed (1.6x) | |
|---|---|---|---|---|
MseI oligo | 1 | 600 | 600 | 1600400 |
H2O | 0.112 | 600 | 67.2179 | 44.28 |
10x T4 Buffer | 0.1 | 600 | 60 | 16040 |
5M NaCl | 0.01 | 600 | 6 | 164 |
1 mg/ml BSA | 0.05 | 600 | 30 | 8020 |
T4 DNA ligase | 0.1675 | 600 | 100.5 | 26867 |
Total | 1.4 | 600 | 840 | 2240560 |
Add 1 ul of EcoR1 Adaptors with 96-channel and Track which template plate gets which MID plate.
...