...
Reagent | ul/rxn | rxns | ul needed (1.5x) |
10x T4 Buffer | 1.15 | 480400 | 690460 |
5M NaCl | 0.12 | 480400 | 7248 |
1 mg/ml BSA | 0.6 | 480400 | 360240 |
H2O | 0.73 | 480400 | 438292 |
MseI (enzyme) | 0.12 | 480400 | 7248 |
EcoR1 (enzyme) | 0.28 | 480400 | 168112 |
Total | 3 | 480400 | 1800 1200 |
Tubes:
Reagent | ul/rxn | rxns | ul needed (1.5x) |
10x T4 Buffer | 1.15 | 56 | 69 |
5M NaCl | 0.12 | 56 | 7.2 |
1 mg/ml BSA | 0.6 | 56 | 36 |
H2O | 0.73 | 56 | 43.8 |
MseI (enzyme) | 0.12 | 56 | 7.2 |
EcoR1 (enzyme) | 0.28 | 56 | 16.8 |
Total | 3 | 56 | 180 |
...