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Gregg Randolph

Gregg Randolph

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Reagent

ul/rxn

rxns

ul needed (x 1.6)

5x Iproof buffer

0.425

2721800

136765

10 mM dNTPs

0.4

2721800

128720

Primers

1.33

2721800

4262394

Total

2.155

2721800

6903879

Then continue with the last four unlisted steps for GBS1 from above:

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