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24 ul PNA in 76 ul TE makes 100 ul 12uM PNAs
Dilute DNAs to 10 ng/ul
Use concentrations provided
MasterMix for standard
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
3 | 5X Kapa HiFi Buffer | 150 | 450 |
0.45 | 10M dNTPs | 150 | 68 |
0.3 | Kapa HiFi HotStart DNA Pol | 150 | 45 |
5.25 | HPLC H2O | 150 | 787 |
9 | Total Volume | 150 | 1350 |
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