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Reagent | ul/rxn | rxns | ul needed (1.5x) | |
10x T4 Buffer | 1.15 | 600 | 690 | 437 |
5M NaCl | 0.12 | 600 | 7245.6 | |
1 mg/ml BSA | 0.6 | 600 | 360 | 228 |
H2O | 0.73 | 600 | 438277.4 | |
MseI (enzyme) | 0.12 | 600 | 7245.6 | |
EcoR1 (enzyme) | 0.28 | 600 | 168 | 106.4 |
Total | 3 | 600 | 18001140 |
Add 6 ul template to each plate with Benchsmart. Cover and seal each plate, vortex, centrifuge and incubate at 37C for 8 hours. Followed by 65C in EcoBGC oven for 1 hour.
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