Page Comparison

This was the page for a first attempt at making this library and is superseded by its parent page.

Quantify DNAs on Synergy HTX Plate Reader:

Normalize DNAs on Nimbus:

Had to define these semi skirted plates for the Nimbus. We might want to standardize to full skirted plates. They are less variable.

16S Library Prep:

Swap out KG4 G9’s blank for Mock Community positive control

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Purify samples using GSAF’s second modified MagBead protocol:

Equilibrate Beads to room Temperature

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ITS Library Prep:

Swap out KG4 G9’s blank for Mock Community positive control

...

Purify samples using GSAF’s second modified MagBead protocol:

Equilibrate Beads to room Temperature

...