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Run under standard conditions
Results:
16S Pool: 0.48 nM
ITS Pool: 3.61 nM
Sequencing:
- 1
0.2 nM 16S Pool:
100/nM pool concentration= ul Pool to add 100-ul Pool to add=ul of 10 mM Tris to add
0.2 nM ITS Pool: 5.6 ul ITS Pool + 94.4 ul “10 mM Tris 8.5”
0.1 nM Full Pool: 50 ul from both 0.2 nM Pools
Add 50 ul 0.1 nM Pool to 40 ul “10 mM Tris 8.5” and 10 ul 50 pM PhiX
Remove iSeq100 Cartridge v2 from large freezer #3 36 hours to 1 week before sequencing and place in refrigerator 5 with room around it for full airflow
Remove iSeq 100 i1 Flow Cell from refrigerator 5’s crisper drawer and open white foil pack and allow to equilibrate to RT for 10-15 minutes.
Open “iSeq 100 i1 Reagent Cartridge v2”. Turn on iSeq100, sbsuser password: “GenomeTechnologies”.
Click on “Sequence”. Watch Video. Do what video tells you to do. Follow on screen instructions until run starts.
Results located: Data/SequencingRuns/”folder with applicable date”/Alignment_1/Fastq/*.fastq.gz
Add 5 ul 1 nM Pool to 85 42.2 ul 16S Pool + 57.8 ul “10 mM Tris 8.5”
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