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| Info |
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4-12-2022 Samples have been digested and ligated. They still need to be pooled, PCRed, pooled all together, size selected, qPCRed and bio analyzed. |
Check In Samples Against List from Jill Hamilton
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Pool | Plate1 | Plate2 | Plate3 | Plate4 |
|---|---|---|---|---|
A | RHIR1 | RHIR2 | RHIR3 | RHIR4 |
Pool | Plate1 | Plate2 | Plate3 | Plate4 |
|---|---|---|---|---|
B | RHIR1 | RHIR2 (FLIPPED) | RHIR3 | RHIR4 (FLIPPED) |
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Pool | Plate5 Col 1-3 | Plate5 Col 4-6 | Plate5 Col 7-9 | Plate5 Col 10-12 |
|---|---|---|---|---|
C1 | RHIR5 (Col 1-3) | RHIR5 (Col 4-6) | RHIR5 (Col 7-9) | RHIR5 (Col 10-12) |
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Pool | Plate5 Col 1-3 | Plate5 Col 6-4 | Plate5 Col 7-9 | Plate5 Col 12-10 |
|---|---|---|---|---|
C2 | RHIR5 (Col 1-3) | RHIR5 (Col 6-4) | RHIR5 (Col 7-9) | RHIR5 (Col 12-10) |
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Pool | Plate6 Col 1 | Plate5 Col 2 | Plate5 Col 3 | Plate5 Col 4 |
|---|---|---|---|---|
C3 | RHIR6 Col1 | RHIR6 Col2 | RHIR6 Col3 | RHIR6 Col4 |
Pool | Plate6 Col 1 | Plate5 Col 2 | Plate5 Col 3 | Plate5 Col 4 |
|---|---|---|---|---|
C4 | RHIR6 Col1 | RHIR6 Col2 (FLIPPED) | RHIR6 Col3 | RHIR6 Col4 (FLIPPED) |
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Pool 2 ul from all wells of the 3 final plates into an 8 tube strip. Pool 30 ul from each of these into one tube after mixing via pipette.
Run Final Product on qPCR for check* (Do we still want to do this and the bioanalyzer?)
Result from qPCR check:
HPau_PP_Final:
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