ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
10 | 5X Phusion HF Buffer | 7 | 70070 |
1.5 | 10M dNTPs | 7 | 10.5 |
1 | Kapa HiFi HotStart DNA Pol | 7 | 7 |
2.5 ul | 5 uM F and R FlowCell Primers | 7 | 35 |
17.520 | HPLC H2O | 7140 | 122.5 |
35 | Total Volume | 7 | 1150 |
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