5536 pcrs, 2 plates only get 10 columns
PCR2 Mastermix Preparation:
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
3 | 5X Phusion HF Buffer | 5536 | 16800 |
0.45 | 10M dNTPs | 5536 | 2520 |
0.3 | Kapa HiFi HotStart DNA Pol | 5536 | 1680* |
0.5 ul | 5 uM F and R FlowCell Primers | 5536 | 2800 |
0.75 | HPLC H2O | 5536 | 4200 |
5 | Total Volume | 5536 | 28000 |
...
Use 96 channel to transfer 10ul PCR1 to appropriately labeled PCR2 redos.
qPCR Check:
| View file | ||
|---|---|---|
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qPCR gives a concentration of 12-16 nMoles/ul. Success! We will mail this to Psomagen on Monday.