...
Average results for the following plates (column 3):
4PA3_16S: 4.04 nanomoles
4PA3_ITS: 46.07 nanomoles
4PA4_16S: 71.0 nanomoles
4PA4_ITS: 68.5 nanomoles
4PA5_16S: 10.26 nanomoles
4PA5_ITS: 41.22 nanomoles
4PA6_16S: 11.57 nanomoles
4PA6_ITS: 44.52 nanomoles
Quantities look good for sequencing. The full result report can be viewed below:
| View file | ||
|---|---|---|
|
MasterMix (make for 12 plates in 15mL conical tube)
...
Average results for the following plates (column 3):
4MR1_16S: 92.23 nanomoles
4MR24MR1_ITS: 53.47 nanomoles
4SC14MR2_16S: 105.34 nanomoles
4SC14MR2_ITS: 66.71 nanomoles
4SC1_16S: 46.64 nanomoles
4SC1_ITS: 47.77 nanomoles
4SC2_16S: 65.97 nanomoles
4SC2_ITS: 55.69 nanomoles
Quantities look good for sequencing. The full result report can be viewed below:
| View file | ||
|---|---|---|
|
MasterMix (make for
...
24 plates in 50mL conical tube)
ul/rxn | Reagent | # of rxns | ul needed |
|---|---|---|---|
3 | 5X Kapa HiFi Buffer | 2500 | 7500 |
0.45 | 10M dNTPs | 2500 | 1125 |
0.3 | Kapa HiFi HotStart DNA Pol | 2500 | 750 |
7.25 | HPLC H2O | 2500 | 18125 |
11 | Total Volume | 2500 | 27500 |
...
Average results for the following plates (column 3):
4LVD1_16S: 112.54 nanomoles
4LVD1_ITS: 24.71 nanomoles
4LVD2_16S: 95.57 nanomoles
4LVD2_ITS: 34.28 nanomoles
4LVD3_16S: 112.47 nanomoles
4LVD3_ITS: 101.03 nanomoles
Quantities look good for sequencing. The full result report can be viewed below:
| View file | ||
|---|---|---|
|
qPCR NovaSeq4_Plate5
Make 1:1000 dilutions of column 3 from the PCR plates by adding 1 ul to 999 ul TE in a deep well plate:
Use other half of NS4_Plate 4 dilution plate to do NS4_Plate5 dilution plate.
...
Average results for the following plates (column 3):
4FB1_16S: 28.57 nanomoles
4FB1_ITS: 45.42 nanomoles
4FB2_16S: 70.82 nanomoles
4FB2_ITS: 71.52 nanomoles
4FB3_16S: 73.04 nanomoles
4FB3_ITS: 72.32 nanomoles
Quantities look good for sequencing. The full result report can be viewed below:
| View file | ||
|---|---|---|
|